Shared resources

One of the goals of the Chemistry and Biology of Heparan Sulfate PEG is to provide the broader scientific community with resources to assist in the application of glycosciences to heart, lung and blood research.

We encourage glycoscience researchers to make use of the resources offered by the laboratories involved in the Chemistry and Biology of Heparan Sulfate PEG.

  • Facility for compositional analysis of GAGs using quantitative CE (see Analytical Biochemistry 1993, 213, 120-127; Electrophoresis 2009, 30, 1544-1551; Electrophoresis 2011, 32, 3070-3077)
  • Facility for studying GAG-protein interactions at a molecular level using fluorescence spectroscopy, capillary electrophoresis, surface plasmon resonance and atomic force microscopy (see Journal of Biological Chemistry 1998, 273,7478-7487; Biochemistry 1998, 37, 13033-13041; Methods in Enzymology 2011, 501, 105-137)
  • Availability of a library of sulfated small molecules as mimetic of GAGs for modulation of protein function (see Bioorganic and Medicinal Chemistry 2005, 13, 1783-1789; Tetrahedron Letters 2007, 48, 6754-6758; Journal of Medicinal Chemistry 2011, 54, 6125-6138; Journal of Medicinal Chemistry 2011, 54, 5522-5531)
  • Availability of in silico libraries of GAG structures (disaccharide to hexasaccharide; ~100,000) for computational molecular modeling of GAG-protein interactions (see Journal of Medicinal Chemistry 2006, 49, 3553-3562; ACS Medicinal Chemistry Letters 2010, 1, 281-285)
  • Facility for compositional analysis of GAGs (see Analytical and Bioanalytical Chemistry 2011, 401 (1), 237-244; Analytical and Bioanalytical Chemistry 2011, 399 (2), 559-570; Journal of Biological Chemistry 2009, 284, 25842-25853)
  • Availability of a library of “Click” Xylosides for GAG expression in cells (see Bioorganic and Medicinal Chemistry Letters 2010, 20, 7269-7273; Glycoconjugate Journal 2010, 27 (6), 625-633; Molecular Biosystems 2010, 6, 1800-1802)
  • Purified recombinant chemokines
  • High field NMR (600, 750 and 800 MHz) based on the structural studies of GAG-protein interactions
  • ITC, SPR, fluorescence and ultracentrifugation-based protein-GAG interactions
  • Animal (mouse) models for GAG-chemokine studies on leukocyte recruitment
  • Cellular assays for characterizing chemokine function
  • Allogeneic and xenogeneic models in nonhuman primates (aortic patch, kidney, liver, heart and islet transplantation)
  • In vitro platelet aggregation assays
  • Xenogeneic mixed lymphocyte cultures
  • ELISA and flow cytometry in vitro assays
  • Tissue factor and C-reactive protein expression
  • Mesenchymal stromal cell isolation and culture
  • Adult and neonatal islet isolation and culture
  • Mouse models of islet allo- and xeno-transplantation
  • Xenotransplant models (ex vivo and in vivo, lung, liver, heart)
  • In vitro flow chamber assays
  • ELISA, flow cytometry and colorimetric in vitro assays to quantify platelet activation, thrombin generation and protein C activation
  • Gene expression

Request computational resources

Heparan sulfate virtual libraries in SYBYL mol2 format, configuration files and automation scripts for use with docking and virtual screening programs are freely available from the Chemistry and Biology of Heparan Sulfate PEG. Please fill out the form below and return it to pegadmin@vcu.edu.

Download/print the HSPG PEG request form. Get Adobe Reader

Program investigators

Umesh R. Desai, Ph.D.
urdesai@vcu.edu
Phone: (804) 828-7328

Philip Mosier, Ph.D.
pdmosier@vcu.edu
Phone: (804) 828-7405

Kuberan Balagurunathan, Ph.D.
kuby@pharm.utah.edu
Phone: (801) 647-6700

Krishna Rajarathnam, Ph.D.
krrajara@utmb.edu
Phone: (409) 772-2238

David K.C. Cooper, M.D., Ph.D.
coopdk@pittsburgh.edu
Phone: (412) 383-6961